Analytical HPLC System¶
Peptide Purity Analysis¶
Category¶
Analysis Equipment
Application¶
Purity determination, method development, and quality control
Function¶
Quantify peptide purity and identify impurities in crude and purified samples.
Key Parameters¶
| Parameter | Typical Range |
|---|---|
| Flow rate | 0.5–2.0 mL/min |
| Column ID | 2.1–4.6 mm |
| Column length | 50–250 mm |
| Particle size | 3–5 μm |
| Max pressure | 400–1000 bar |
| Detection | UV-Vis, DAD (diode array) |
Column Selection¶
| Column Type | Application |
|---|---|
| C18 (most common) | General peptide analysis |
| C8 | More hydrophobic peptides |
| C4 | Large peptides and proteins |
| Phenyl | Alternative selectivity |
Method Parameters¶
| Parameter | Typical Value |
|---|---|
| Temperature | 25–40 °C |
| Gradient slope | 1–2% B/min |
| Runtime | 20–40 minutes |
| Detection | 214 nm (primary), 254 nm, 280 nm |
Related QC Tests¶
- Purity assessment
- Impurity profiling
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Method development for preparative scale
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Peptide Purity Analysis — Review the section above for key parameters, methods, and quality criteria.
- Column Selection — Review the section above for key parameters, methods, and quality criteria.
- Method Parameters — Review the section above for key parameters, methods, and quality criteria.
Key Takeaways¶
- Peptide Purity Analysis — Review the section above for key parameters, methods, and quality criteria.
- Application — Review the section above for key parameters, methods, and quality criteria.
- Key Parameters — Review the section above for key parameters, methods, and quality criteria.
- Column Selection — Review the section above for key parameters, methods, and quality criteria.
🔗 Related: Preparative HPLC | HPLC Analysis | Purity Analysis