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LC-MS System

Liquid Chromatography-Mass Spectrometry

Category

Analysis Equipment

Application

Identity confirmation and purity assessment

Function

Combines HPLC separation with mass detection for molecular weight confirmation of peptide peaks.

Key Parameters

Parameter Typical Range
Mass range (m/z) 100–3000 (standard), up to 6000 (extended)
Resolution Unit resolution (standard quadrupole)
Ionization ESI (electrospray ionization)
Detection modes Full scan, SIM, MRM

LC-MS Workflow

  1. HPLC separates peptide components
  2. ESI ionizes peptide molecules
  3. Mass analyzer measures m/z
  4. Software deconvolutes to calculate molecular weight

Typical Results

Measurement Expected
Mass accuracy ±0.5 Da
Match to theoretical ±1 Da (confirms identity)
Adducts Na⁺, K⁺, TFA adducts common
  • Identity confirmation
  • Deletion sequence detection

  • Impurity identification

  • Liquid Chromatography-Mass Spectrometry — Review the section above for key parameters, methods, and quality criteria.

  • Typical Results — Review the section above for key parameters, methods, and quality criteria.

Key Takeaways

  • Liquid Chromatography-Mass Spectrometry — Review the section above for key parameters, methods, and quality criteria.
  • Application — Review the section above for key parameters, methods, and quality criteria.
  • Key Parameters — Review the section above for key parameters, methods, and quality criteria.
  • LC-MS Workflow — Review the section above for key parameters, methods, and quality criteria.

🔗 Related: Mass Spectrometer | LC-MS Testing | Mass Confirmation | Peptide Quality Control Guide