Purification Workflow Diagram¶

Preparative HPLC Peptide Purification¶

flowchart TD
    A[Crude Peptide] --> B[Dissolve in<br/>Mobile Phase A];
    B --> C[Filter<br/>0.45 μm];
    C --> D[Equilibrate Column];
    D --> E[Load Sample];
    E --> F[Gradient Elution<br/>5-60% B];
    F --> G[UV Detection<br/>214 nm];
    G --> H[Peak Detection];
    H --> I[Collect Fractions];
    I --> J[Analytical HPLC<br/>Screen Fractions];
    J --> K{Purity ≥98%?};
    K -->|Yes| L[Pool Pure<br/>Fractions];
    K -->|No| M{Purity ≥90%?};
    M -->|Yes| N[Re-purify];
    N --> F;
    M -->|No| O[Discard];
    L --> P[Lyophilize];
    P --> Q[Final Purified<br/>Peptide];

Purity Decision Flow¶

• Preparative HPLC Peptide Purification — Review the section above for key parameters, methods, and quality criteria.
• Purity Decision Flow — Review the section above for key parameters, methods, and quality criteria.

Key Takeaways¶

• Preparative HPLC Peptide Purification — Review the section above for key parameters, methods, and quality criteria.
• Purity Decision Flow — Review the section above for key parameters, methods, and quality criteria.

🔗 Related: Purification Process | Preparative HPLC | HPLC Analysis