HATU — High-Efficiency Coupling Reagent¶
For Difficult Peptide Couplings¶
Definition¶
HATU (O-(7-azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate) is a high-reactivity coupling reagent for difficult couplings.
Specifications¶
| Parameter | Typical Value |
|---|---|
| Purity | ≥99% |
| Appearance | White crystalline powder |
| Storage | -20 °C, desiccated |
| CAS number | 148893-10-1 |
When to Use HATU¶
| Scenario | Recommendation |
|---|---|
| Sterically hindered AAs (Val, Ile, Thr) | HATU recommended |
| His coupling | HATU (lower racemization) |
| Long sequences (>20 AA) | Double coupling + HATU |
| Routine couplings | HBTU sufficient |
Typical Conditions¶
| Parameter | Value |
|---|---|
| Equivalents | 2.9 eq (relative to AA) |
| Base | DIEA (6 eq) |
| Activation time | 2–3 minutes |
| Coupling time | 30–60 minutes |
Advantages¶
- Higher coupling efficiency than HBTU
- Lower racemization for sensitive amino acids
- Faster reaction kinetics
Disadvantages¶
- Significantly more expensive than HBTU
-
Used selectively, not for routine couplings
-
For Difficult Peptide Couplings — Review the section above for key parameters, methods, and quality criteria.
- When to Use HATU — See the section for detailed parameters and specifications.
- Typical Conditions — Review the section above for key parameters, methods, and quality criteria.
Key Takeaways¶
- For Difficult Peptide Couplings — Review the section above for key parameters, methods, and quality criteria.
- Definition — Review the section above for key parameters, methods, and quality criteria.
- Specifications — Review the section above for key parameters, methods, and quality criteria.
- When to Use HATU — See the section above for detailed parameters and specifications.
🔗 Related: HBTU | DIC | Coupling Reaction